Bioinformatics in practice, Faroe Islands 2024
This is the home page for week 45+46 of the "Bioinformatics in practice" course, Faroe Islands 2024. These five days are taught by Henrik Nielsen and Bent Petersen.
Monday November 4
Morning: Introduction, plain text files and taxonomy databases
Lectures:
- Introduction to bioinformatics and computers - Henrik
- Evolution & taxonomy - Bent
Exercises:
Afternoon: GenBank
Lecture: Biological information, DNA, sequencing, and GenBank - Henrik
Handout exercise: "Base-calling" — Print version (PDF) / On-screen version (PDF)
Materials:
Exercise: Using the GenBank database
Wednesday November 6
Morning: Translation and UniProt
Lecture: Proteins: data and databases - Henrik
Materials:
- Virtual Ribosome — software article (PDF)
- Proteins, levels of structure (PDF)
- Eukaryotic gene structure overview (PDF)
Exercises:
Afternoon: Protein structure, PDB & PyMOL
Lecture: Protein 3D structure - Bent
Software for installation: PyMOL
- Note: you will need a license file which we will provide via Zoom.
Exercises:
Thursday November 7
Morning: Pairwise alignment
Lecture: Pairwise alignment - Henrik
Handout exercise: Alignment scores
Exercise: Pairwise alignment
Wrap-up: Pairwise alignment revisited
Afternoon: Sequence database searching with BLAST
Lecture: Introduction to BLAST - Bent
Exercise: Exercise: BLAST
Wrap-up: BLASTN vs BLASTP
Monday November 11
Morning: Sequence information and logo plots
Lecture: Sequence information and logo plots - Henrik
Materials: "Information theory primer" (PDF)
Handout exercise: How to construct sequence logos
Exercise: DNA and Peptide LOGOs
Afternoon: Profile searching with PSI-BLAST
Lecture: PSI-BLAST - Bent
Exercise: PSI-BLAST
Wednesday November 13
Morning: Multiple alignments
Lecture: Multiple Alignments - Henrik
Materials: RevTrans (article, PDF)
Exercise: Multiple Alignments
Afternoon: Phylogenetic trees
Lecture: Phylogenetic trees - Bent
Handout exercise: Reconstruction of distance tree
Exercise: Phylogeny