NCBI Blast:sp|O43292|GPAA1_HUMAN GPI-anchor transamidase

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Search Parameters
Search parameter name	Search parameter value
Program	blastp
Word size	3
Expect value	10
Hitlist size	500
Gapcosts	11,1
Matrix	BLOSUM62
Filter string	F
Genetic Code	1
Window Size	40
Threshold	11
Composition-based stats	1

Database
Database parameter name	Database parameter value
Posted date	Sep 30, 2025 3:22 AM
Number of letters	44,538,409
Number of sequences	91,647
Entrez query	Includes: Trypanosoma (taxid:5690) Excludes: None

Karlin-Altschul statistics
Params	Ungapped	Gapped
Lambda	0.323919	0.267
K	0.138361	0.0506795
H	0.420947	0.14
Alpha	0.7916	1.9
Alpha_v	4.96466	42.6028
Sigma		43.6362

Your search is limited to records include: Trypanosoma (taxid:5690)

Job Title

sp|O43292|GPAA1_HUMAN GPI-anchor transamidase ...

RID

H3EGCKDB016 Search expires on 11-11 17:10 pm

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Results for

Program

PSI-BLAST Iteration 1

Citation
Reference
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference - composition-based statistics starting in round 2
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.

Database

refseq_protein

See details

Query ID

lcl|Query_2207285

Description

sp|O43292|GPAA1_HUMAN GPI-anchor transamidase component GPAA1 OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3 ...

Molecule type

amino acid

Query Length

621

Other reports

Distance tree of results Multiple alignment MSA viewer

Filter Results

Run PSI-Blast iteration 2

Descriptions

14 sequences selected

, Reading indexes 1-5, displaying indexes 1-5

Sequences with E-value BETTER than threshold

select all
2 sequences selected

PSI-BLAST iteration 1

Sequences producing significant alignments with E-value BETTER than threshold
Select for downloading or viewing reports	Description	Scientific Name	Common Name	Taxid	Max Score	Total Score	Query Cover	E value	Per. Ident	Acc. Len	Accession	Select for PSI blast	Used to build PSSM	Newly added
1Select seq ref\|XP_067191308.1\|	GPI transamidase component Gaa1 [Trypanosoma melophagium]	Trypanosoma melophagium	NA	715481	48.2	48.2	27%	4e-05	25.99%	463	XP_067191308.1(scored below threshold on previous iteration)	Select seq XP_067191308.1 for PSI blast
2Select seq ref\|XP_028878215.1\|	putative GPI transamidase component GAA1 [Trypanosoma theileri]	Trypanosoma theileri	NA	67003	42.0	42.0	27%	0.003	24.71%	463	XP_028878215.1(scored below threshold on previous iteration)	Select seq XP_028878215.1 for PSI blast

Sequences with E-value WORSE than threshold

select all
12 sequences selected

PSI-BLAST iteration 1

Sequences with E-value WORSE than threshold
Select for downloading or viewing reports	Description	Scientific Name	Common Name	Taxid	Max Score	Total Score	Query Cover	E value	Per. Ident	Acc. Len	Accession	Select for PSI blast
3Select seq ref\|XP_806050.1\|	hypothetical protein, conserved [Trypanosoma cruzi]	Trypanosoma cruzi	NA	5693	34.0	34.0	12%	1.1	31.65%	782	XP_806050.1(scored below threshold on previous iteration)	Select seq XP_806050.1 for PSI blast
4Select seq ref\|XP_028880603.1\|	uncharacterized protein TM35_000271270 [Trypanosoma theileri]	Trypanosoma theileri	NA	67003	34.0	34.0	11%	1.2	30.56%	780	XP_028880603.1(scored below threshold on previous iteration)	Select seq XP_028880603.1 for PSI blast
5Select seq ref\|XP_816950.1\|	hypothetical protein, conserved [Trypanosoma cruzi]	Trypanosoma cruzi	NA	5693	33.6	33.6	12%	1.6	30.38%	783	XP_816950.1(scored below threshold on previous iteration)	Select seq XP_816950.1 for PSI blast
6Select seq ref\|XP_029236587.1\|	putative GPI transamidase component GAA1 [Trypanosoma rangeli]	Trypanosoma rangeli	NA	5698	33.2	33.2	27%	1.6	25.99%	469	XP_029236587.1(scored below threshold on previous iteration)	Select seq XP_029236587.1 for PSI blast
7Select seq ref\|XP_029226505.1\|	putative GPI transamidase component GAA1 [Trypanosoma conorhini]	Trypanosoma conorhini	NA	83891	33.2	33.2	27%	1.8	26.52%	469	XP_029226505.1(scored below threshold on previous iteration)	Select seq XP_029226505.1 for PSI blast
8Select seq ref\|XP_009312321.1\|	hypothetical protein DQ04_05201050 [Trypanosoma grayi]	Trypanosoma grayi	NA	71804	33.2	33.2	8%	1.9	46.00%	755	XP_009312321.1(scored below threshold on previous iteration)	Select seq XP_009312321.1 for PSI blast
9Select seq ref\|XP_009307093.1\|	hypothetical protein DQ04_00351080 [Trypanosoma grayi]	Trypanosoma grayi	NA	71804	32.8	32.8	7%	3.0	34.88%	807	XP_009307093.1(scored below threshold on previous iteration)	Select seq XP_009307093.1 for PSI blast
10Select seq ref\|XP_822281.1\|	GPI transamidase component GAA1 [Trypanosoma brucei brucei TREU927]	Trypanosoma brucei brucei TREU927	NA	185431	32.0	32.0	24%	4.3	24.26%	461	XP_822281.1(scored below threshold on previous iteration)	Select seq XP_822281.1 for PSI blast
11Select seq ref\|XP_011777197.1\|	GPI transamidase component GAA1 [Trypanosoma brucei gambiense DAL972]	Trypanosoma brucei gambiense DAL972	NA	679716	32.0	32.0	24%	4.3	24.26%	461	XP_011777197.1(scored below threshold on previous iteration)	Select seq XP_011777197.1 for PSI blast
12Select seq ref\|XP_067078424.1\|	GPI transamidase component GAA1 [Trypanosoma equiperdum]	Trypanosoma equiperdum	NA	5694	32.0	32.0	24%	4.3	24.26%	461	XP_067078424.1(scored below threshold on previous iteration)	Select seq XP_067078424.1 for PSI blast
13Select seq ref\|XP_067193532.1\|	AARP2CN [Trypanosoma melophagium]	Trypanosoma melophagium	NA	715481	31.6	31.6	12%	5.4	27.85%	780	XP_067193532.1(scored below threshold on previous iteration)	Select seq XP_067193532.1 for PSI blast
14Select seq ref\|XP_029236815.1\|	uncharacterized protein TraAM80_06501 [Trypanosoma rangeli]	Trypanosoma rangeli	NA	5698	30.9	30.9	12%	6.9	32.10%	242	XP_029236815.1(scored below threshold on previous iteration)	Select seq XP_029236815.1 for PSI blast

- hover to see the title
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- Show Conserved Domains
- Alignment Scores
- < 40
- 40 - 50
- 50 - 80
- 80 - 200
- >= 200
- Help
  The graphic is an overview of the database sequences aligned to the query sequence. These are represented horizontal bars colored coded by score and showing the extent of the alignment on the query sequence. Separate aligned regions on the same database sequence are connected by a thin grey line. Mousing over an alignment shows the database sequence title. Clicking an alignment displays a box with more details about the alignment and link to the sequence alignment itself in the Alignments section of the report.
- 14 sequences selected
- Help
Putative conserved domains have been detected, click on the image below for detailed results.

Distribution of the top 14 Blast Hits on 14 subject sequences

Query

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- Alignment view
- CDS feature
- Line length:
- Help
  Alignment view: Choose how to view alignments. The default "pairwise" view shows how each subject sequence aligns individually to the query sequence. The "query-anchored" view shows how all subject sequences align to the query sequence. For each view type, you can choose to show "identities" (matching residues) as letters or dots. more...
  
  CDS feature: Show annotated coding region and translation. more...
  
  Line length: Number of letters to show on one line in an alignment.
- 14 sequences selected
- Help
Loading alignment... for sequences ref|XP_067191308.1|,ref|XP_028878215.1|,ref|XP_806050.1|,ref|XP_028880603.1|,ref|XP_816950.1| Reading indexes 1-5

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GPI transamidase component Gaa1 [Trypanosoma melophagium]
Sequence ID: XP_067191308.1Length: 463Number of Matches: 1

Related Information
NewGenome Data Viewer-aligned genomic context

Range 1: 137 to 302GenPept Graphics Next Match Previous Match First Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame

48.2 bits(114) 4e-05() Composition-based stats. 46/177(26%) 77/177(43%) 19/177(10%)

Features:

Query 219 IQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGL----LCT---LQGK 271 +++A+ + +SS L V G NG PN DLLNL + ++ GL LC Sbjct 137 VRSALIINISSLNTRKLCFGVYGKNGMQPNQDLLNLAVSMAKEWGLKVDILCQNPYTTYS 196 Query 272 LQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQY-KY 330 L + + LQT L+ A P H +F + + +L++ + S Y Sbjct 197 LSRSIYDH---YVTALQTALI-----APQYPQAWH-IFRSHGISSLSISTVKSDNGYDST 247 Query 331 DLVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGL 387 D ++ LE + L++L ER H S +++ + +V + G +LLV L Sbjct 248 DAYSIAAMLEQIIATLSYLDERFHHSTSVWVPLSVKHYVE--YDIAQFGIILLVASL 302

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putative GPI transamidase component GAA1 [Trypanosoma theileri]
Sequence ID: XP_028878215.1Length: 463Number of Matches: 1

Related Information
Gene-associated gene details
AlphaFold Structure-3D structure displays
NewGenome Data Viewer-aligned genomic context

Range 1: 137 to 302GenPept Graphics Next Match Previous Match First Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame

42.0 bits(98) 0.003() Composition-based stats. 43/174(25%) 78/174(44%) 13/174(7%)

Features:

Query 219 IQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGL----LCTLQGKLQP 274 +++A+ + +SS +L V G NG PN DL N+ + ++ GL LC Sbjct 137 VRSALVINVSSLNTRNLCFGVYGKNGMQPNQDLFNVAVSMAKEWGLKVDVLCQNPYTTYS 196 Query 275 EDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQY-KYDLV 333 + + + LQT L+ A P H F + + +L++ + S Y + D Sbjct 197 LSRSMYEHYITALQTALI-----APQYPQPWHS-FRSHGISSLSISTVKSDENYNRSDAF 250 Query 334 AVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGL 387 ++ LE + L++L ER H S +++ ++ + I + G +LLV L Sbjct 251 SMVAMLEQIIATLSYLDERFHHSTSVWVPLSVTHY--IEYDIAQFGIMLLVASL 302

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hypothetical protein, conserved [Trypanosoma cruzi]
Sequence ID: XP_806050.1Length: 782Number of Matches: 1

Related Information
Gene-associated gene details
AlphaFold Structure-3D structure displays
NewGenome Data Viewer-aligned genomic context

Range 1: 176 to 247GenPept Graphics Next Match Previous Match First Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame

34.0 bits(77) 1.1() Composition-based stats. 25/79(32%) 36/79(45%) 12/79(15%)

Features:

Query 298 ASGRPH-GSHGL----FLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLER 352 ASG G GL F R + AL +G+ S +V V + LE K + R Sbjct 176 ASGTTWFGDIGLCITDFTRSMITALNAQGVPS-------IVVVLQNLETFAEKQRQKVLR 228 Query 353 LHQSFFLYLLPGLSRFVSI 371 +HQ +F +LP +R +I Sbjct 229 VHQRYFTSVLPDTTRTFAI 247

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uncharacterized protein TM35_000271270 [Trypanosoma theileri]
Sequence ID: XP_028880603.1Length: 780Number of Matches: 1

Related Information
Gene-associated gene details
AlphaFold Structure-3D structure displays
NewGenome Data Viewer-aligned genomic context

Range 1: 183 to 247GenPept Graphics Next Match Previous Match First Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame

34.0 bits(77) 1.2() Composition-based stats. 22/72(31%) 35/72(48%) 11/72(15%)

Features:

Query 304 GSHGL----FLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFL 359 G GL F R + +L +G+ + + V + L+ K H + R+HQ +FL Sbjct 183 GDIGLCITDFTRDLITSLNAQGVPT-------VTVVLQNLDTFSEKQRHKVLRVHQRYFL 235 Query 360 YLLPGLSRFVSI 371 +LP +R VSI Sbjct 236 SVLPETTRVVSI 247

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hypothetical protein, conserved [Trypanosoma cruzi]
Sequence ID: XP_816950.1Length: 783Number of Matches: 1

Related Information
Gene-associated gene details
AlphaFold Structure-3D structure displays
NewGenome Data Viewer-aligned genomic context

Range 1: 176 to 247GenPept Graphics Next Match Previous Match First Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame

33.6 bits(76) 1.6() Composition-based stats. 24/79(30%) 37/79(46%) 12/79(15%)

Features:

Query 298 ASGRPH-GSHGL----FLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLER 352 ASG+ G GL F R + AL +G+ S +V V + LE K + R Sbjct 176 ASGKTWFGDIGLCITDFTRSMITALNAQGVPS-------IVVVLQNLETFAEKQRQQVLR 228 Query 353 LHQSFFLYLLPGLSRFVSI 371 +HQ +F +LP ++ +I Sbjct 229 VHQRYFTSVLPDTTKTFAI 247

Alignment statistics for match #1
Score	Expect	Method	Identities	Positives	Gaps	Frame
48.2 bits(114)	4e-05()	Composition-based stats.	46/177(26%)	77/177(43%)	19/177(10%)

Alignment statistics for match #1
Score	Expect	Method	Identities	Positives	Gaps	Frame
42.0 bits(98)	0.003()	Composition-based stats.	43/174(25%)	78/174(44%)	13/174(7%)

Alignment statistics for match #1
Score	Expect	Method	Identities	Positives	Gaps	Frame
34.0 bits(77)	1.1()	Composition-based stats.	25/79(32%)	36/79(45%)	12/79(15%)

Alignment statistics for match #1
Score	Expect	Method	Identities	Positives	Gaps	Frame
34.0 bits(77)	1.2()	Composition-based stats.	22/72(31%)	35/72(48%)	11/72(15%)

Alignment statistics for match #1
Score	Expect	Method	Identities	Positives	Gaps	Frame
33.6 bits(76)	1.6()	Composition-based stats.	24/79(30%)	37/79(46%)	12/79(15%)

Taxonomy

14 sequences selected
Help

Lineage

Lineage Report
Organism	Blast Name	Score	Number of Hits	Description
Trypanosoma	kinetoplastids		14
.Trypanosoma melophagium	kinetoplastids	48.2	2	Trypanosoma melophagium hits
.Trypanosoma theileri	kinetoplastids	42.0	2	Trypanosoma theileri hits
.Trypanosoma cruzi	kinetoplastids	34.0	2	Trypanosoma cruzi hits
.Trypanosoma rangeli	kinetoplastids	33.2	2	Trypanosoma rangeli hits
.Trypanosoma conorhini	kinetoplastids	33.2	1	Trypanosoma conorhini hits
.Trypanosoma grayi	kinetoplastids	33.2	2	Trypanosoma grayi hits
.Trypanosoma brucei brucei TREU927	kinetoplastids	32.0	1	Trypanosoma brucei brucei TREU927 hits
.Trypanosoma brucei gambiense DAL972	kinetoplastids	32.0	1	Trypanosoma brucei gambiense DAL972 hits
.Trypanosoma equiperdum	kinetoplastids	32.0	1	Trypanosoma equiperdum hits

Organism

Organism Report
Description	Score	E value	Accession
Trypanosoma melophagium [kinetoplastids] Next Previous First
GPI transamidase component Gaa1 [Trypanosoma melophagium]	48.2	4e-05	XP_067191308
AARP2CN [Trypanosoma melophagium]	31.6	5.4	XP_067193532
Trypanosoma theileri [kinetoplastids] Next Previous First
putative GPI transamidase component GAA1 [Trypanosoma theileri]	42.0	0.003	XP_028878215
uncharacterized protein TM35_000271270 [Trypanosoma theileri]	34.0	1.2	XP_028880603
Trypanosoma cruzi [kinetoplastids] Next Previous First
hypothetical protein, conserved [Trypanosoma cruzi]	34.0	1.1	XP_806050
hypothetical protein, conserved [Trypanosoma cruzi]	33.6	1.6	XP_816950
Trypanosoma rangeli [kinetoplastids] Next Previous First
putative GPI transamidase component GAA1 [Trypanosoma rangeli]	33.2	1.6	XP_029236587
uncharacterized protein TraAM80_06501 [Trypanosoma rangeli]	30.9	6.9	XP_029236815
Trypanosoma conorhini [kinetoplastids] Next Previous First
putative GPI transamidase component GAA1 [Trypanosoma conorhini]	33.2	1.8	XP_029226505
Trypanosoma grayi [kinetoplastids] Next Previous First
hypothetical protein DQ04_05201050 [Trypanosoma grayi]	33.2	1.9	XP_009312321
hypothetical protein DQ04_00351080 [Trypanosoma grayi]	32.8	3.0	XP_009307093
Trypanosoma brucei brucei TREU927 [kinetoplastids] Next Previous First
GPI transamidase component GAA1 [Trypanosoma brucei brucei TREU927]	32.0	4.3	XP_822281
Trypanosoma brucei gambiense DAL972 [kinetoplastids] Next Previous First
GPI transamidase component GAA1 [Trypanosoma brucei gambiense DAL972]	32.0	4.3	XP_011777197
Trypanosoma equiperdum [kinetoplastids] Next Previous First
GPI transamidase component GAA1 [Trypanosoma equiperdum]	32.0	4.3	XP_067078424

Taxonomy

Taxonomy Report
Taxonomy	Number of hits	Number of Organisms	Description
Trypanosoma	14	9
. Trypanosoma melophagium	2	1	Trypanosoma melophagium hits
. Trypanosoma with unspecified subgenus	5	3
.. Trypanosoma theileri	2	1	Trypanosoma theileri hits
.. Trypanosoma conorhini	1	1	Trypanosoma conorhini hits
.. Trypanosoma grayi	2	1	Trypanosoma grayi hits
. Trypanosoma cruzi	2	1	Trypanosoma cruzi hits
. Trypanosoma rangeli	2	1	Trypanosoma rangeli hits
. Trypanozoon	3	3
.. Trypanosoma brucei	2	2
... Trypanosoma brucei brucei TREU927	1	1	Trypanosoma brucei brucei TREU927 hits
... Trypanosoma brucei gambiense DAL972	1	1	Trypanosoma brucei gambiense DAL972 hits
.. Trypanosoma equiperdum	1	1	Trypanosoma equiperdum hits

Notice

Important update

Filter Results

Run PSI-Blast iteration 2

Distribution of the top 14 Blast Hits on 14 subject sequences

Notice

Important update

Filter Results

Run PSI-Blast iteration 2

Sequences producing significant alignments