22126 - Next Generation Sequencing Analysis

Introduction to Next-Generation Sequencing Analysis, 5 ECTS

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DTU's Studies Handbook about #36626 DTU's Studies Handbook about #36826

Program 2024

Program 2023

Program 2022

Program 2021

Program 2020

Program 2019

The next course will be held in January 2024, the course runs every day for a three weeks period. The course consists of lectures, exercises and project work. The prerequisites are basic knowledge of molecular biology. Additionally, because many of the exercises will be performed using command-line basic knowledge of how to navigate in a Unix/Linux environment is a plus, but not required, and will be taught on the first day of the course. The course will be conducted in English.

For more information, please contact Gabriel Renaud, or alternatively click on the links above.

Learning objectives

A student who has met the objectives of the course will be able to:

• Describe the applications of the different NGS technologies, including the weakness and strengths of the approaches.
• Explain the steps involved in a general NGS data analysis.
• Explain key theoretical concepts of alignment and de novo assembly.
• Use programs in Unix for analysis of NGS data.
• Synthesize and formulate a project and relevant question within the field.
• Independently perform a basic NGS data analysis.
• Apply analytical and reflective skills in analyzing results from individual steps and the final project.
• Cooperate in groups for project work and communicate results on a poster.

Textbook

There are no textbooks, and none is necessary for following the course. Articles will be available during the course to support understanding of the subjects.

If you need to brush up on your biology
Here is a very nice YouTube video by thebiologyprimer: The Central Dogma: DNA to proteins (an animated lecture video)

Registration:

If you are a DTU student please sign up via standard procedures. If you are not enrolled at the Technical University of Denmark, sign up as a guest student (see link below).

Further questions regarding registration may be addressed to: phdcourses@dtu.dk

• General information for guest students
• The course exercises are made to be run on the pupil system and not on your own computer, but you do need to use your own laptop to log in to the pupil servers. To log in you need an ssh client program. If you are using MS windows, go to the mobaxterm website and install MobaXterm. If you are using a Mac you must have X11 or XQuartz installed, if you have Mac OSX > 10.6 you can install XQuartz from http://xquartz.macosforge.org/landing/. Linux should have ssh built-in. A more comprehensive guide can be found at http://teaching.healthtech.dtu.dk/22113/index.php/Course_preparation