22126 - Next Generation Sequencing Analysis
This is also course 22176, 36626 and 36826.
5 points course, June 2019
The next course will be held in June 2019 (6th of June 2019 - 27th of June 2019), the course runs every day for a three weeks period in June 2019 in Room 062 in Building 208. The course consists of lectures, exercises and project work. Prerequisistes are basic knowledge of Molecular Biology. Additionally because the many of the exercises will be performed using command-line basic knowledge of how to navigate in a Unix/Linux environment is a plus, but not required, and will be tought on the first day of the course. The course will be conducted in English.
For more information, please contact Gisle Vestergaard, or alternatively click on the links above.
A student who has met the objectives of the course will be able to:
- Describe the applications of the different NGS technologies, including the weakness and strengths of the approaches.
- Explain the steps involved in a general NGS data analysis.
- Explain key theoretical concepts of alignment and de novo assembly.
- Apply programs in Unix for analysis of NGS data.
- Synthesize and formulate a project and relevant question within the field.
- Independently perform a basic NGS data analysis.
- Apply analytical and reflective skills in analyzing results from individual steps and the final project.
- Cooperate in groups for project work and communicate results on a poster.
There are no textbook, and none is necessary for following the course. Articles will be available during the course to support understanding of the subjects.
If you need to brush up on your biology
Here is a very nice lecture series by Dr Norman Cohen from Open University named DNA, RNA and protein formation.
If you are a DTU student please sign up via standard procedures. If you are not enrolled at the Technical University of Denmark, sign up as a guest student (see link below).
Further questions regarding registration may be addresed to Pia Christoffersen
- General information for guest students
- The course exercises are made to be run on the Computerome supercomputer and not on your own computer, but you do need to use your own laptop to log in the Computerome system. To log in you need a ssh client program. If you are using MS windows, go to the mobaxterm website and install MobaXterm. If you are using a Mac you must have X11 or XQuartz installed, if you have Mac OSX > 10.6 you can install XQuartz from http://xquartz.macosforge.org/landing/. Linux should have ssh built-in. A more comprehensive guide can be found at http://teaching.healthtech.dtu.dk/36610/index.php/Course_preparation